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Product Name
VAMP3/Cellubrevin antibody
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Description
VAMP3/Cellubrevin Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human kidney tissue. Positive WB detected in HEK-293 cells, MCF7 cells, mouse brain tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 11-17 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CEB antibody; Cellubrevin antibody; SYB3 antibody; Synaptobrevin 3 antibody; VAMP 3 antibody; VAMP3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VAMP3/Cellubrevin recombinant protein (Accession Number: NM_004781). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116706(VAMP3 antibody) at dilution of 1:1000
IP Result of anti-VAMP3 (IP:Catalog No:116706, 4ug; Detection:Catalog No:116706 1:1000) with HEK-293 cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116706(VAMP3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116706(VAMP3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.
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References
- Bian F, Yang X, Zhou F. C-reactive protein promotes atherosclerosis by increasing LDL transcytosis across endothelial cells. British journal of pharmacology. 171(10):2671-84. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"