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Product Name
VAMP1 antibody
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Description
VAMP1 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive WB detected in human brain tissue. Observed molecular weight by Western-blot: 13 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686H12131 antibody; SYB1 antibody; Synaptobrevin 1 antibody; VAMP 1 antibody; VAMP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VAMP1 recombinant protein (Accession Number: NM_199245). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116704(VAMP1 antibody) at dilution of 1:500
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:116704(VAMP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VAMP1, also named as synaptobrevin 1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP1 is a part of the SNARE (soluble NSF-attachment protein receptor) complex. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP1 is involved in synaptic vesicle exocytosis, a fundamental step in neurotransmitter release.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"