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Product Name
USP9X antibody
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Description
USP9X Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, human brain tissue, K-562 cells. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 260-290 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme FAF X antibody; DFFRX antibody; FAF antibody; FAM antibody; Fat facets in mammals antibody; hFAM antibody; Ubiquitin thiolesterase FAF X antibody; USP9 antibody; USP9X antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001039590). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116692(USP9X antibody) at dilution of 1:500
IP Result of anti-USP9X (IP:Catalog No:116692, 5ug; Detection:Catalog No:116692 1:500) with HEK-293 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:116692(USP9X Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:116692(USP9X Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116692(USP9X Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"