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Product Name
USP49 antibody
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Description
USP49 Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue. Positive WB detected in MCF7 cells. Observed molecular weight by Western-blot: 79-85 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 49 antibody; Ubiquitin thiolesterase 49 antibody; USP49 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP49 recombinant protein (Accession Number: NM_018561). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116687(USP49 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human stomach using Catalog No:116687(USP49 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:116687(USP49 antibody) at dilution of 1:50 (under 40x lens)
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Background
USPs are cysteine proteases that vary greatly in size and structural complexity. Outside of the core catalytic domain, USPs exhibit N-terminal and C-terminal extensions that have been proposed to play roles in determining cellular localization and substrate specificity of these enzymes(PMID:14715245). USP49(Ubiquitin carboxyl-terminal hydrolase 49) belongs to this family. It has 2 isoforms produced by alternative splicing.
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