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Product Name
USP20 antibody
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Description
USP20 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 110-115 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 20 antibody; hVDU2 antibody; KIAA1003 antibody; LSFR3A antibody; Ubiquitin thiolesterase 20 antibody; USP20 antibody; VDU2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP20 recombinant protein (Accession Number: NM_006676). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116598(USP20 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human kidney using Catalog No:116598(USP20 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:116598(USP20 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-USP20 (IP:Catalog No:116598, 4ug; Detection:Catalog No:116598 1:500) with HeLa cells lysate 1200ug.
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Background
USP20(ubiquitin carboxyl-terminal hydrolase 20) is also named as KIAA1003, LSFR3A, VDU2 and belongs to the peptidase C19 family and the USP20/USP33 subfamily. It can specifically deubiquitinate and stabilize HIF1A and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF). It also plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2.
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