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Product Name
UQCRFS1 antibody
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Description
UQCRFS1 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human heart tissue. Positive WB detected in MCF-7 cells, A2780 cells, HEK-293 cells, HeLa cells, MCF7 cells, mouse brain tissue, mouse heart tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Complex III subunit 5 antibody; Complex III subunit IX antibody; Rieske iron sulfur protein antibody; RIP1 antibody; RIS1 antibody; RISP antibody; UQCR5 antibody; UQCRFS1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UQCRFS1 recombinant protein (Accession Number: NM_006003). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116637(UQCRFS1 Antibody) at dilution of 1:2000
IP Result of anti-UQCRFS1 (IP:Catalog No:116637, 4ug; Detection:Catalog No:116637 1:1000) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:116637(UQCRFS1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:116637(UQCRFS1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.
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References
- Sun S, Han Y, Liu J. Trichostatin A targets the mitochondrial respiratory chain, increasing mitochondrial reactive oxygen species production to trigger apoptosis in human breast cancer cells. PloS one. 9(3):e91610. 2014.
- Zhang J, Wang C, Chen X. EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. The EMBO journal. 34(23):2953-70. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"