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Product Name
UPF3A antibody
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Description
UPF3A Rabbit Polyclonal antibody. Positive WB detected in human testis tissue, HeLa cells, Jurkat cells. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human heart tissue. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hUpf3 antibody; HUPF3A antibody; RENT3A antibody; UPF3 antibody; UPF3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UPF3A recombinant protein (Accession Number: NM_080687). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:116626(UPF3A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:116626(UPF3A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:116626(UPF3A antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116626(UPF3A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
UPF3A represents UPF3 regulator of nonsense transcripts homolog A (yeast)
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References
- Feng D, Su RC, Zou L, Triggs-Raine B, Huang S, Xie J. Increase of a group of PTC(+) transcripts by curcumin through inhibition of the NMD pathway. Biochimica et biophysica acta. 1849(8):1104-15. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"