UPF1 antibody

Cat.#: 116625

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Product Information

  • Product Name
    UPF1 antibody
  • Documents
  • Description
    UPF1 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive IF detected in HepG2 cells, MCF-7 cells. Positive WB detected in HepG2 cells, Raji cells. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 130 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATP dependent helicase RENT1 antibody; FLJ43809 antibody; FLJ46894 antibody; HUPF1 antibody; KIAA0221 antibody; NORF1 antibody; pNORF1 antibody; RENT1 antibody; UPF1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UPF1 recombinant protein (Accession Number: NM_002911). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116625(UPF1 antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116625(UPF1 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116625(UPF1 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116625(UPF1 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116625(UPF1 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116625(UPF1 Antibody) at dilution of 1:50

    Immunofluorescent analysis of HepG2 cells using Catalog No:116625(UPF1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells using Catalog No:116625(UPF1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-UPF1 (IP:Catalog No:116625, 4ug; Detection:Catalog No:116625 1:500) with Raji cells lysate 2000ug.

    IP Result of anti-UPF1 (IP:Catalog No:116625, 4ug; Detection:Catalog No:116625 1:500) with Raji cells lysate 2000ug.

  • Background
    UPF1 represents UPF1 regulator of nonsense transcripts homolog (yeast)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"