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Product Name
UGT8 antibody
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Description
UGT8 Rabbit Polyclonal antibody. Positive WB detected in rat brain tissue, mouse brain tissue, mouse cerebellum tissue. Positive IP detected in rat brain tissue. Positive IHC detected in human brain tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 61kd
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ceramide galactosyltransferase antibody; Cerebroside synthase antibody; CGT antibody; UDP glycosyltransferase 8 antibody; UGT4 antibody; UGT8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UGT8 recombinant protein (Accession Number: XM_047416134). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116561(UGT8 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:116561(UGT8 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:116561(UGT8 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-UGT8 (IP:Catalog No:116561, 4ug; Detection:Catalog No:116561 1:1000) with rat brain tissue lysate 4000ug.
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Background
UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.
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References
- Sun Z, Shi S, Li H. Evaluation of resveratrol sensitivities and metabolic patterns in human and rat glioblastoma cells. Cancer chemotherapy and pharmacology. 72(5):965-73. 2013.
- Shu XH, Wang LL, Li H. Diffusion Efficiency and Bioavailability of Resveratrol Administered to Rat Brain by Different Routes: Therapeutic Implications. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics. 12(2):491-501. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"