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Product Name
UFC1 antibody
- Documents
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Description
UFC1 Rabbit Polyclonal antibody. Positive WB detected in mouse pancreas tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 21kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HSPC155 antibody; UFC1 antibody; Ufm1 conjugating enzyme 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UFC1 recombinant protein (Accession Number: XM_017001451). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:116550(UFC1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:116550(UFC1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116550(UFC1 antibody) at dilution of 1:100 (under 40x lens)
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Background
The function of UFC1 (ubiquitin fold modifier conjugating enzyme 1) has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein. Catalog#15783-1-AP specially recognises the 21 kDa protein.
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References
- Gavin JM, Hoar K, Xu Q. Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway. The Journal of biological chemistry. 289(33):22648-58. 2014.
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