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Product Name
UBE2A antibody
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Description
UBE2A Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in HEK-293 cells, COLO 320 cells, human kidney tissue, human placenta tissue. Observed molecular weight by Western-blot: 17 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HHR6A antibody; HR6A antibody; RAD6 homolog A antibody; RAD6A antibody; UBC2 antibody; UBE2A antibody; Ubiquitin carrier protein A antibody; Ubiquitin protein ligase A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBE2A recombinant protein (Accession Number: NM_003336). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116515(UBE2A antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116515(UBE2A antibody) at dilution of 1:50 (under 10x lens)
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Background
UBE2A(Ubiquitin-conjugating enzyme E2 A) serves as the cognate E2-conjugating enzyme. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions. UBE2A, UBE2B regulates TP53 levels in a "yin-yang" manner through a combination of two distinct mechanisms in mammalian cells(PMID:22083959). This protein has 3 isoforms produced by alternative splicing.
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References
- Fiesel FC, Moussaud-Lamodière EL, Ando M, Springer W. A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. Journal of cell science. 127(Pt 16):3488-504. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"