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UBE1 antibody

Cat.#: 116513

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Special Price 239.0 USD

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Product Information

  • Product Name
    UBE1 antibody
  • Documents
  • Description
    UBE1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HL-60 cells, HEK-293 cells, HeLa cells, mouse colon tissue, mouse spleen tissue, rat spleen tissue. Observed molecular weight by Western-blot: 118 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    A1S9 antibody; A1S9T antibody; A1ST antibody; AMCX1 antibody; GXP1 antibody; Protein A1S9 antibody; SMAX2 antibody; UBA1 antibody; UBA1A antibody; UBE1 antibody; UBE1X antibody; Ubiquitin activating enzyme E1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UBE1 recombinant protein (Accession Number: XM_017029781). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:116513(UBE1 antibody) at dilution of 1:500

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:116513(UBE1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116513(UBE1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116513(UBE1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116513(UBE1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116513(UBE1 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    UBE1(Ubiquitin-activating enzyme E1) is also named as A1S9T, UBE1 and belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation and initiates the activation and conjugation of ubiquitin-like proteins. Defects in UBE1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2).
  • References
    • Yang LS, Xu XE, Liu XP. iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p. Journal of proteomics. 77:480-91. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"