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Product Name
UBC9-Specific antibody
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Description
UBC9-Specific Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in HEK-293 cells, human testis tissue. Observed molecular weight by Western-blot: 18kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C358B7.1 antibody; UBE2I antibody; P18 antibody; SUMO conjugating enzyme UBC9 antibody; SUMO protein ligase antibody; UBC9 antibody; UBCE9 antibody; UBE2I antibody; Ubiquitin carrier protein 9 antibody; Ubiquitin carrier protein I antibody; Ubiquitin protein ligase I antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003345). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116511(UBE2I-Specific antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116511(UBE2I-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116511(UBE2I-Specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:116511(UBE2I-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
1X10^6 HepG2 cells were stained with 0.2ug UBE2I-Specific antibody (Catalog No:116511, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"