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Product Name
TWIST1-specific antibody
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Description
TWIST1-specific Rabbit Polyclonal antibody. Positive IP detected in MDA-MB-453s cells. Positive WB detected in MDA-MB-453s cells, A549 cells, mouse brain tissue, Raji cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ACS3 antibody; bHLHa38 antibody; BPES2 antibody; BPES3 antibody; H twist antibody; SCS antibody; TWIST antibody; twist homolog 1 (Drosophila) antibody; Twist related protein 1 antibody; TWIST1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TWIST1-specific recombinant protein (Accession Number: XM_011515496). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:116502(TWIST1 Antibody) at dilution of 1:600
IP Result of anti-TWIST1 (IP:Catalog No:116502, 4ug; Detection:Catalog No:116502 1:500) with MDA-MB-453s cells lysate 920ug.
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Background
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with twist1 protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"