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Product Name
TST antibody
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Description
TST Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, HeLa cells, HepG2 cells, human kidney tissue, liver tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
RDS antibody; Rhodanese antibody; Thiosulfate sulfurtransferase antibody; TST antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TST recombinant protein (Accession Number: NM_003312). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
WB result of anti-TST(Catalog No:116373) in Liver by Dr. Seow Chong Lee.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116373(TST antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116373(TST antibody) at dilution of 1:50 (under 40x lens)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116373(TST Antibody) at dilution of 1:600
IP Result of anti-TST (IP:Catalog No:116373, 4ug; Detection:Catalog No:116373 1:300) with HEK-293 cells lysate 2800ug.
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Background
Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.
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References
- Roman HB, Hirschberger LL, Krijt J, Valli A, Kožich V, Stipanuk MH. The cysteine dioxgenase knockout mouse: altered cysteine metabolism in nonhepatic tissues leads to excess H2S/HS(-) production and evidence of pancreatic and lung toxicity. Antioxidants & redox signaling. 19(12):1321-36. 2013.
- Jurkowska H, Roman HB, Hirschberger LL. Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate. Amino acids. 46(5):1353-65. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"