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Product Name
TRPC6 antibody
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Description
TRPC6 Rabbit Polyclonal antibody. Positive IP detected in mouse lung tissue. Positive WB detected in mouse brain, COLO 320 cells, mouse lung tissue. Positive FC detected in A549 cells. Positive IHC detected in human placenta tissue. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, IHC, WB, IP, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
FLJ11098 antibody; FLJ14863 antibody; FSGS2 antibody; Transient receptor protein 6 antibody; TRP 6 antibody; TRP6 antibody; TRPC6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRPC6 recombinant protein (Accession Number: NM_004621). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: N/AIP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116409(TRPC6 antibody) at dilution of 1:500
IP Result of anti-TRPC6 (IP:Catalog No:116409, 4ug; Detection:Catalog No:116409 1:300) with mouse lung tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:116409(TRPC6 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:116409(TRPC6 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 A549 cells were stained with 0.2ug TRPC6 antibody (Catalog No:116409, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
TRPC6 represents transient receptor potential cation channel, subfamily C, member 6
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References
- Riazanski V, Gabdoulkhakova AG, Boynton LS. TRPC6 channel translocation into phagosomal membrane augments phagosomal function. Proceedings of the National Academy of Sciences of the United States of America. 112(47):E6486-95. 2015.
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