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Product Name
TRIM9 antibody
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Description
TRIM9 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HepG2 cells. Observed molecular weight by Western-blot: 90 kDa, 61 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0282 antibody; RING finger protein 91 antibody; RNF91 antibody; SPRING antibody; TRIM9 antibody; tripartite motif containing 9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM9 recombinant protein (Accession Number: NM_052978). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116325(TRIM9 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:116325(TRIM9 antibody) at dilution of 1:100 (under 25x lens)
IP Result of anti-TRIM9 (IP:Catalog No:116325, 4ug; Detection:Catalog No:116325 1:1000) with mouse brain tissue lysate 4000ug.
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Background
TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms produced by alternative splicing.
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References
- Tanji K, Kamitani T, Mori F, Kakita A, Takahashi H, Wakabayashi K. TRIM9, a novel brain-specific E3 ubiquitin ligase, is repressed in the brain of Parkinson's disease and dementia with Lewy bodies. Neurobiology of disease. 38(2):210-8. 2010.
- Mishima C, Kagara N, Matsui S. Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patients. SpringerPlus. 4:635. 2015.
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