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Product Name
TRIM54 antibody
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Description
TRIM54 Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human skeletal muscle tissue. Positive IHC detected in human heart. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
TRIM54 antibody; MuRF3 antibody; RING finger protein 30 antibody; tripartite motif containing 54 antibody; RNF30 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM54 recombinant protein (Accession Number: XM_024453010). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116320(TRIM54 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:116320(TRIM54 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:116320(TRIM54 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using TRIM54 antibody Catalog No:116320 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
TRIM54(Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30.The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion.It is expressed in heart and skeletal muscle specifically (PMID:11243782).It has two isforms with the molecular weight of 40KDa and 45KDa.It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer(PMID:15967462).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"