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Product Name
TRIM32 antibody
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Description
TRIM32 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human lymphoma tissue, human colon tissue. Observed molecular weight by Western-blot: 65-72 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
72 kDa Tat interacting protein antibody; BBS11 antibody; HT2A antibody; LGMD2H antibody; TATIP antibody; TRIM32 antibody; tripartite motif containing 32 antibody; Zinc finger protein HT2A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM32 recombinant protein (Accession Number: NM_001379050). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116311(TRIM32 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116311(TRIM32 antibody) at dilution of 1:50 (under 10x lens)
IF result (cytoplasm stain) of anti-BBS11 (Catalog No:116311; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.
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Background
TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).
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References
- Kudryashova E, Wu J, Havton LA, Spencer MJ. Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Human molecular genetics. 18(7):1353-67. 2009.
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