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Product Name
TRAPPC3 antibody
- Documents
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Description
TRAPPC3 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, HEK-293 cells, HepG2 cells, mouse small intestine tissue, PC-3 cells. Positive IHC detected in human placenta tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 20-22 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BET3 antibody; BET3 homolog antibody; TRAPPC3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRAPPC3 recombinant protein (Accession Number: NM_014408). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116344(TRAPPC3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116344(TRAPPC3 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using TRAPPC3 antibody Catalog No:116344 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TRAPPC3 (IP:Catalog No:116344, 3ug; Detection:Catalog No:116344 1:800) with mouse liver tissue lysate 4000ug.
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Background
TRAPPC3 (trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines. (15728249, 21273506, 23394947)
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References
- Bassik MC, Kampmann M, Lebbink RJ. A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. Cell. 152(4):909-22. 2013.
- Westlake CJ, Baye LM, Nachury MV. Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome. Proceedings of the National Academy of Sciences of the United States of America. 108(7):2759-64. 2011.
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