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Product Name
TRAPB/SSR2 antibody
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Description
TRAPB/SSR2 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, L02 cells, mouse liver tissue. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human pancreas tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 22-25kd
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686F19123 antibody; HSD25 antibody; SSR beta antibody; SSR2 antibody; TLAP antibody; TRAP BETA antibody; TRAPB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRAPB/SSR2 recombinant protein (Accession Number: NM_003145). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116340(TRAPB, SSR2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116340(TRAPB, SSR2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116340(TRAPB, SSR2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:116340(TRAPB, SSR2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug TRAPB, SSR2 antibody (Catalog No:116340, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"