-
Product Name
transgelin/SM22-specific antibody
- Documents
-
Description
transgelin/SM22-specific Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human colon tissue, human kidney tissue. Positive WB detected in human colon tissue, mouse colon tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 22 kDa
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
SM22 antibody; SM22 alpha antibody; SM22a antibody; SM22alpha antibody; Smooth muscle protein 22 alpha antibody; TAGLN antibody; TAGLN1 antibody; transgelin antibody; TRANSGELIN 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003186). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:100-1:2000
-
Validations
human colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:116334(TRANSGELIN-1-specific antibody) at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:116334(TRANSGELIN-1-specific Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:116334(TRANSGELIN-1-specific Antibody) at dilution of 1:200 (under 40x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116334(TRANSGELIN-1-specific Antibody) at dilution of 1:2000 (under 10x lens).
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116334(TRANSGELIN-1-specific Antibody) at dilution of 1:2000 (under 40x lens).
-
Background
The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is speific to transgelin 1. It does not cross-react with transgelin 2 and 3.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"