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Product Name
Transgelin 2 antibody
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Description
Transgelin 2 Mouse Monoclonal antibody. Positive IHC detected in human colon cancer tissue, human colon tissue. Positive WB detected in K562 cells, A549 cells, HEK-293 cells, MCF-7 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 22 kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
SM22 beta antibody; SM22beta antibody; TAGLN2 antibody; Transgelin 2 antibody; Transgelin 2/TAGLN2 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Transgelin 2 recombinant protein (Accession Number: NM_003564). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107639(TAGLN2 antibody) at dilution of 1:1000
IP Result of anti-TAGLN2 (IP:Catalog No:107639, 4ug; Detection:Catalog No:107639 1:1000) with HEK-293 cells lysate 1000ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107639(TAGLN2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107639(TAGLN2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.
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