-
Product Name
TPT1 antibody
- Documents
-
Description
TPT1 Rabbit Polyclonal antibody. Positive IP detected in human placenta tissue. Positive WB detected in mouse placenta tissue, A431 cells, HepG2 cells, MCF7 cells, mouse testis tissue, PC-3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 22-25 kDa
-
Tested applications
ELISA, WB, IP, IHC
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
FLJ27337 antibody; Fortilin antibody; Histamine releasing factor antibody; HRF antibody; p02 antibody; p23 antibody; TCTP antibody; TPT1 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TPT1 recombinant protein (Accession Number: NM_003295). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
mouse placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:116220(TPT1 antibody) at dilution of 1:800
IP Result of anti-TPT1 (IP:Catalog No:116220, 4ug; Detection:Catalog No:116220 1:500) with human placenta tissue lysate 1520ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:116220(TPT1 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:116220(TPT1 Antibody) at dilution of 1:200 (under 40x lens).
-
Background
The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.
-
References
- Staflin K, Zuchner T, Honeth G, Darabi A, Lundberg C. Identification of proteins involved in neural progenitor cell targeting of gliomas. BMC cancer. 9:206. 2009.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"