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Product Name
TOP2B antibody
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Description
TOP2B Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in Hela cells, HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, K-562 cells. Observed molecular weight by Western-blot: 180kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DNA topoisomerase 2 beta antibody; TOP2B antibody; top2beta antibody; TOPIIB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001068). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116248(TOP2B antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:116248(TOP2B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TOP2B antibody Catalog No:116248 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TOP2B (IP:Catalog No:116248, 4ug; Detection:Catalog No:116248 1:300) with HepG2 cells lysate 3200ug.
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Background
TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.
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References
- Minajigi A, Froberg JE, Wei C. Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. Science (New York, N.Y.). 349(6245):. 2015.
- Lu C, Liu X, Liu C. Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells. Toxicological sciences : an official journal of the Society of Toxicology. 147(2):588-606. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"