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Product Name
TNIP1 antibody
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Description
TNIP1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IF detected in Hela cells. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 72kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
ABIN 1 antibody; HIV 1 Nef interacting protein antibody; hVAN antibody; KIAA0113 antibody; NAF1 antibody; Nef assOCiated factor 1 antibody; Nip40 1 antibody; TNFAIP3 interacting protein 1 antibody; TNIP1 antibody; VAN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TNIP1 recombinant protein (Accession Number: NM_001252391). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116144(TNIP1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116144(TNIP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116144(TNIP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TNIP1 antibody Catalog No:116144 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TNIP1 (IP:Catalog No:116144, 4ug; Detection:Catalog No:116144 1:700) with HeLa cells lysate 2000ug.
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Background
TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs. TNIP1 has three isoforms with MW 66 kDa aand 71-72 kDa.
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References
- Olarerin-George AO, Anton L, Hwang YC, Elovitz MA, Hogenesch JB. A functional genomics screen for microRNA regulators of NF-kappaB signaling. BMC biology. 11:19. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"