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Product Name
TMS1 antibody
- Documents
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Description
TMS1 Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells, A549 cells, Raji cells, U-937 cells. Positive IP detected in HL-60 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 22-25 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ASC antibody; CARD5 antibody; hASC antibody; PYCARD antibody; PYD and CARD domain containing antibody; TMS antibody; TMS 1 antibody; TMS1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TMS1 recombinant protein (Accession Number: NM_145183). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:116279(TMS1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116279(TMS1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-TMS1 (IP:Catalog No:116279, 4ug; Detection:Catalog No:116279 1:2000) with HL-60 cells lysate 4000ug.
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Background
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
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References
- Parsons MJ, Patel P, Brat DJ, Colbert L, Vertino PM. Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells. Cancer research. 69(5):1706-11. 2009.
- Kapoor-Vazirani P, Kagey JD, Powell DR, Vertino PM. Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity. Cancer research. 68(16):6810-21. 2008.
- Rajandram R, Pat BK, Li J, Johnson DW, Gobe GC. Expression of apoptotic tumour necrosis factor receptor-associated factor, caspase recruitment domain and cell death-inducing DFF-45 effector genes in therapy-treated renal cell carcinoma. Nephrology (Carlton, Vic.). 14(2):205-12. 2009.
- Kapoor-Vazirani P, Kagey JD, Vertino PM. SUV420H2-mediated H4K20 trimethylation enforces RNA polymerase II promoter-proximal pausing by blocking hMOF-dependent H4K16 acetylation. Molecular and cellular biology. 31(8):1594-609. 2011.
- Liu W, Luo Y, Dunn JH, Norris DA, Dinarello CA, Fujita M. Dual role of apoptosis-associated speck-like protein containing a CARD (ASC) in tumorigenesis of human melanoma. The Journal of investigative dermatology. 133(2):518-27. 2013.
- Zhang X, Ibrahim E, de Rivero Vaccari JP. Involvement of the inflammasome in abnormal semen quality of men with spinal cord injury. Fertility and sterility. 99(1):118-24. 2013.
- Liu W, Yin Y, Zhou Z, He M, Dai Y. OxLDL-induced IL-1 beta secretion promoting foam cells formation was mainly via CD36 mediated ROS production leading to NLRP3 inflammasome activation. Inflammation research : official journal of the European Histamine Research Society ...
- Kapoor-Vazirani P, Vertino PM. A dual role for the histone methyltransferase PR-SET7/SETD8 and histone H4 lysine 20 monomethylation in the local regulation of RNA polymerase II pausing. The Journal of biological chemistry. 289(11):7425-37. 2014.
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