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Product Name
TMEM165 antibody
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Description
TMEM165 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HeLa cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 35-40 kDa
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Tested applications
ELISA, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
TMEM165 antibody; TMPT27 antibody; TPARL antibody; transmembrane protein 165 antibody; Transmembrane protein PT27 antibody; Transmembrane protein TPARL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TMEM165 recombinant protein (Accession Number: BC003545). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116102(TMEM165 antibody) at dilution of 1:3000
Immunofluorescent analysis of HepG2 cells using Catalog No:116102(TMEM165 Antibody) at dilution of 1:100 and Rhodamine-labeled goat anti-rabbit IgG
IP Result of anti-TMEM165 (IP:Catalog No:116102, 4ug; Detection:Catalog No:116102 1:1000) with HeLa cells lysate 3440ug.
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Background
Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.
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References
- Reinhardt TA, Lippolis JD, Sacco RE. The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1). Biochemical and biophysical research communications. 445(2):417-21. 2014.
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