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Product Name
TMEM106B antibody
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Description
TMEM106B Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, A549 cells, HeLa cells, human placenta tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human brain tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 31-35kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ11273 antibody; TMEM106B antibody; transmembrane protein 106B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TMEM106B recombinant protein (Accession Number: NM_001134232). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116091(TMEM106B antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human brain using Catalog No:116091(TMEM106B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:116091(TMEM106B antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-TMEM106B (IP:Catalog No:116091, 3ug; Detection:Catalog No:116091 1:1000) with mouse brain tissue lysate 6000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116091(TMEM106B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. TMEM106B can be showed as 31-40kDa and 60-75kDa (Glycosylated or Dimer) form in western blot test.
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References
- Chen-Plotkin AS, Unger TL, Gallagher MD. TMEM106B, the risk gene for frontotemporal dementia, is regulated by the microRNA-132/212 cluster and affects progranulin pathways. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(33):11213-27. 2012.
- Satoh J, Kino Y, Kawana N. TMEM106B expression is reduced in Alzheimer's disease brains. Alzheimer's research & therapy. 6(2):17. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"