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Product Name
TMED9 antibody
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Description
TMED9 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, mouse brain tissue, mouse lung tissue, mouse pancreas tissue, mouse skin tissue, rat liver tissue. Positive IP detected in mouse liver tissue. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Glycoprotein 25L2 antibody; GMP25 antibody; GP25L2 antibody; HSGP25L2G antibody; TMED9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TMED9 recombinant protein (Accession Number: NM_017510). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116088(TMED9 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:116088(TMED9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116088(TMED9 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TMED9 antibody Catalog No:116088 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TMED9 (IP:Catalog No:116088, 4ug; Detection:Catalog No:116088 1:500) with mouse liver tissue lysate 4000ug.
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Background
TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).
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