-
Product Name
TLE3 antibody
- Documents
-
Description
TLE3 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, A431 cells, HeLa cells, human lung tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 83kd
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ESG antibody; ESG3 antibody; FLJ39460 antibody; GRG3 antibody; HsT18976 antibody; KIAA1547 antibody; TLE3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TLE3 recombinant protein (Accession Number: BC015729). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116069(TLE3 antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:116069(TLE3 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:116069(TLE3 Antibody) at dilution of 1:50
IP Result of anti-TLE3 (IP:Catalog No:116069, 4ug; Detection:Catalog No:116069 1:300) with HeLa cells lysate 1600ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:116069(TLE3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
-
Background
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane
-
References
- Villanueva CJ, Vergnes L, Wang J. Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs. Cell metabolism. 17(3):423-35. 2013.
- Ortega FJ, Serrano M, Rodriguez-Cuenca S. Transducin-like enhancer of split 3 (TLE3) in adipose tissue is increased in situations characterized by decreased PPARγ gene expression. Journal of molecular medicine (Berlin, Germany). 93(1):83-92. 2015.
- Villanueva CJ, Waki H, Godio C. TLE3 is a dual-function transcriptional coregulator of adipogenesis. Cell metabolism. 13(4):413-27. 2011.
- Naderi A, Meyer M, Dowhan DH. Cross-regulation between FOXA1 and ErbB2 signaling in estrogen receptor-negative breast cancer. Neoplasia (New York, N.Y.). 14(4):283-96. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"