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Product Name
TIM22 antibody
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Description
TIM22 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human lung tissue. Positive IP detected in mouse liver tissue. Positive WB detected in HepG2 cells, human liver tissue, human lung tissue, human ovary cancer, human stomach tissue, human testis tissue, mouse liver tissue, mouse ovary tissue, mouse testis tissue. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Testis expressed sequence 4 antibody; TEX4 antibody; TIM22 antibody; TIMM22 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TIM22 recombinant protein (Accession Number: NM_013337). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116126(TIMM22 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:116126(TIMM22 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116126(TIMM22 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-TIMM22 (IP:Catalog No:116126, 4ug; Detection:Catalog No:116126 1:1000) with mouse liver tissue lysate 4000ug.
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Background
TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.
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References
- Richter-Dennerlein R, Korwitz A, Haag M. DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Cell metabolism. 20(1):158-71. 2014.
- Li HY, Zhang LK, Zhu XJ. Analysis of EV71 infection progression using triple-SILAC-based proteomics approach. Proteomics. 15(21):3629-43. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"