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Product Name
TFF2 antibody
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Description
TFF2 Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue, human stomach tissue, mouse liver tissue, mouse pancreas tissue. Positive WB detected in human stomach tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SML1 antibody; Spasmolysin antibody; Spasmolytic polypeptide antibody; TFF2 antibody; trefoil factor 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TFF2 recombinant protein (Accession Number: NM_005423). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:116011(TFF2 antibody) at dilution of 1:100
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:116011(TFF2 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:116011(TFF2 antibody) at dilution of 1:50 (under 10x lens)
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Background
The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system.
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References
- Delker DA, McGettigan BM, Kanth P. RNA sequencing of sessile serrated colon polyps identifies differentially expressed genes and immunohistochemical markers. PloS one. 9(2):e88367. 2014.
- Jiang P, Yu G, Zhang Y. Promoter hypermethylation and downregulation of trefoil factor 2 in human gastric cancer. Oncology letters. 7(5):1525-1531. 2014.
- Seishima R, Wada T, Tsuchihashi K. Ink4a/Arf-Dependent Loss of Parietal Cells Induced by Oxidative Stress Promotes CD44-Dependent Gastric Tumorigenesis. Cancer prevention research (Philadelphia, Pa.). 8(6):492-501. 2015.
- Ando K, Fujiya M, Konishi H. Heterogeneous Nuclear Ribonucleoprotein A1 Improves the Intestinal Injury by Regulating Apoptosis Through Trefoil Factor 2 in Mice with Anti-CD3-induced Enteritis. Inflammatory bowel diseases. 21(7):1541-52. 2015.
- Yamaguchi J, Liss AS, Sontheimer A. Pancreatic duct glands (PDGs) are a progenitor compartment responsible for pancreatic ductal epithelial repair. Stem cell research. 15(1):190-202. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"