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Product Name
TERF2IP antibody
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Description
TERF2IP Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive IF detected in Hela cells. Positive FC detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, HT-1080 cells, human placenta tissue, K-562 cells. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IF, IHC, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DRIP5 antibody; hRap1 antibody; RAP1 antibody; RAP1 homolog antibody; TERF2IP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TERF2IP recombinant protein (Accession Number: NM_018975). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115948(TERF2IP antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using TERF2IP antibody Catalog No:115948 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TERF2IP (IP:Catalog No:115948, 3ug; Detection:Catalog No:115948 1:800) with HeLa cells lysate 2720ug.
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:115948(TERF2IP Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug TERF2IP antibody (Catalog No:115948, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
TERF2IP represents telomeric repeat binding factor 2, interacting protein
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