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Product Name
TDP-43 (full length) antibody
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Description
TDP-43 (full length) Rabbit Polyclonal antibody. Positive FC detected in SH-SY5Y cells. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue. Positive WB detected in HeLa cells, human heart tissue, K-562 cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, IHC, IP, WB, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALS10 antibody; TAR DNA binding protein antibody; TAR DNA binding protein 43 antibody; TARDBP antibody; TDP 43 antibody; TDP43 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TDP-43 (full length) recombinant protein (Accession Number: BC001487). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115927(TDP-43 Antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115927(TARDBP antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-TARDBP (IP:Catalog No:115927, 3ug; Detection:Catalog No:115927 1:1500) with K-562 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115927(TARDBP antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:115927(TDP-43 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 SH-SY5Y cells were stained with 0.2ug TDP-43 antibody (Catalog No:115927, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 18280-1-AP is a rabbit polyclonal antibody raised against the full-length of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.
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