TCTN2 antibody

TCTN2 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

TCTN2 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human liver tissue. Positive IP detected in RPE1 cells(siRNA).

ELISA, IP, IF, IHC

Human,Mouse,Rat; other species not tested.

C12orf38 antibody; FLJ12975 antibody; TCTN2 antibody; TECT2 antibody; Tectonic 2 antibody; tectonic family member 2 antibody

Rabbit IgG

This antibody was obtained by immunization of TCTN2 recombinant protein (Accession Number: NM_024809). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.

• Sang L, Miller JJ, Corbit KC. Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways. Cell. 145(4):513-28. 2011.
• Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS. A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Nature genetics. 43(8):776-84. 2011.
• Wang WJ, Tay HG, Soni R. CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nature cell biology. 15(6):591-601. 2013.