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Product Name
TCP1 antibody
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Description
TCP1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in MCF7 cells, HeLa cells, Jurkat cells, mouse heart tissue. Positive IHC detected in human colon tissue, human colon cancer tissue, human liver cancer tissue. Positive IF detected in Hela cells, MCF-7 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IF, IHC, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CCT alpha antibody; CCT1 antibody; CCTa antibody; D6S230E antibody; t complex 1 antibody; TCP 1 alpha antibody; TCP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TCP1 recombinant protein (Accession Number: NM_030752). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115915(TCP1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon using Catalog No:115915(TCP1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using TCP1 antibody Catalog No:115915 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TCP1 (IP:Catalog No:115915, 4ug; Detection:Catalog No:115915 1:300) with HeLa cells lysate 1600ug.
1X10^6 HeLa cells were stained with 0.2ug TCP1 antibody (Catalog No:115915, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
TCP1 represents t-complex 1
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References
- Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"