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Product Name
Syntaxin 6 antibody
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Description
Syntaxin 6 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human prostate cancer tissue. Positive IF detected in murine BM-DC, murine Spleen-DC. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
STX6 antibody; syntaxin 6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Syntaxin 6 recombinant protein (Accession Number: NM_005819). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
IF result of anti-STX6(Catalog No:115799) in Murine BM-DC by Dr. LOREDANA SAVEANU.
IF result of anti-STX6(Catalog No:115799) in Murine Spleen-DC by Dr. LOREDANA SAVEANU.
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115799(STX6 antibody) at dilution of 1:2000
IP Result of anti-STX6 (IP:Catalog No:115799, 3ug; Detection:Catalog No:115799 1:1000) with mouse brain tissue lysate 7000ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:115799(STX6 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:115799(STX6 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.
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References
- Weimershaus M, Maschalidi S, Sepulveda F, Manoury B, van Endert P, Saveanu L. Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation. Journal of immunology (Baltimore, Md. : 1950). 188(4):1840-6. 2012.
- Wang L, Zhan Y, Song E. HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus. Protein & cell. 2(1):74-85. 2011.
- Saveanu L, Carroll O, Weimershaus M. IRAP identifies an endosomal compartment required for MHC class I cross-presentation. Science (New York, N.Y.). 325(5937):213-7. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"