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Product Name
Syntaxin 12 antibody
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Description
Syntaxin 12 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue, human brain tissue, human kidney tissue, human pancreas tissue. Positive WB detected in mouse brain tissue, BxPC-3 cells, human brain tissue, human heart tissue, mouse heart tissue. Observed molecular weight by Western-blot: 33kd,39kd,66kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
STX12 antibody; STX13 antibody; STX14 antibody; syntaxin 12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Syntaxin 12 recombinant protein (Accession Number: NM_177424). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunofluorescent analysis of HepG2 cells, using STX12 antibody Catalog No:115792 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with 0.2ug STX12 antibody (Catalog No:115792, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115792(STX12 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115792(STX12 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115792(STX12 antibody) at dilution of 1:100 (under 40x lens)
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Background
Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).
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References
- Lu Y, Zhang Z, Sun D, Sweeney ST, Gao FB. Syntaxin 13, a genetic modifier of mutant CHMP2B in frontotemporal dementia, is required for autophagosome maturation. Molecular cell. 52(2):264-71. 2013.
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