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Product Name
SYNJ1 antibody
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Description
SYNJ1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, NIH/3T3 cells. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 140 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
INPP5G antibody; KIAA0910 antibody; synaptojanin 1 antibody; SYNJ1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SYNJ1 recombinant protein (Accession Number: BC136603). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115784(SYNJ1 Antibody) at dilution of 1:4000
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:115784(SYNJ1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues.(PMID:10801423).
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