-
Product Name
Syndecan-3 antibody
- Documents
-
Description
Syndecan-3 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, Jurkat cells, L02 cells, MCF7 cells, mouse kidney tissue, mouse lung tissue, rat kidney tissue, SH-SY5Y cells. Positive IP detected in mouse lung tissue, A549 cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in PC-12 cells, MCF-7 cells. Observed molecular weight by Western-blot: 60-70 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
KIAA0468 antibody; N syndecan antibody; SDC3 antibody; SDCN antibody; SYND3 antibody; syndecan 3 antibody; Syndecan3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Syndecan-3 recombinant protein (Accession Number: BC013974). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:115781(SDC3 antibody) at dilution of 1:1000
IP Result of anti-SDC3 (IP:Catalog No:115781, 4ug; Detection:Catalog No:115781 1:1000) with mouse lung tissue lysate 4000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115781(SDC3 antibody) at dilution of 1:100 (under 25x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed PC-12 cells using Catalog No:115781(SDC3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
-
References
- Reijnders CM, Waaijer CJ, Hamilton A. No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas. The American journal of pathology. 177(4):1946-57. 2010.
- Wu ZS, Pandey V, Wu WY, Ye S, Zhu T, Lobie PE. Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma. BMC cancer. 13:34. 2013.
- van Oosterwijk JG, Meijer D, van Ruler MA. Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets. The American journal of pathology. 182(4):1347-56. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"