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Product Name
SUZ12 antibody
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Description
SUZ12 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, MCF7 cells. Observed molecular weight by Western-blot: 80-90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ChET 9 protein antibody; CHET9 antibody; JJAZ1 antibody; Joined to JAZF1 protein antibody; KIAA0160 antibody; Polycomb protein SUZ12 antibody; SUZ12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015355). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115753(SUZ12 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:115753(SUZ12 Antibody) at dilution of 1:50
IP Result of anti-SUZ12 (IP:Catalog No:115753, 4ug; Detection:Catalog No:115753 1:500) with HeLa cells lysate 3200ug.
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Background
SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.
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