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Product Name
STMN3 antibody
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Description
STMN3 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, rat brain tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 21-25kd
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
SCG10 like protein antibody; SCLIP antibody; Stathmin 3 antibody; stathmin like 3 antibody; STMN3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STMN3 recombinant protein (Accession Number: NM_015894). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115727(STMN3 antibody) at dilution of 1:2000
IP Result of anti-STMN3 (IP:Catalog No:115727, 3ug; Detection:Catalog No:115727 1:2000) with mouse brain tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115727(STMN3 antibody) at dilution of 1:50 (under 10x lens)
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Background
Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.
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References
- Xie X, Bartholomeusz C, Ahmed AA. Bisphosphorylated PEA-15 sensitizes ovarian cancer cells to paclitaxel by impairing the microtubule-destabilizing effect of SCLIP. Molecular cancer therapeutics. 12(6):1099-111. 2013.
- Singer S, Malz M, Herpel E. Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer. Cancer research. 69(6):2234-43. 2009.
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