Product Name

STMN2 antibody

Documents

Description

STMN2 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human medulloblastoma tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, mouse cerebellum tissue. Observed molecular weight by Western-blot: 18-21kd

Tested applications

ELISA, IHC, IP, WB, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Protein SCG10 antibody; SCG10 antibody; SCGN10 antibody; SGC10 antibody; Stathmin 2 antibody; stathmin like 2 antibody; STMN2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of STMN2 recombinant protein (Accession Number: NM_007029). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115726(STMN2 antibody) at dilution of 1:2000

IP Result of anti-STMN2 (IP:Catalog No:115726, 3ug; Detection:Catalog No:115726 1:1000) with mouse brain tissue lysate 9500ug.

Immunohistochemical of paraffin-embedded human medulloblastoma using Catalog No:115726(STMN2 antibody) at dilution of 1:100 (under 10x lens)

Immunofluorescent analysis of SH-SY5Y cells using Catalog No:115726(STMN2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer’s disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

References

Shin JE, Miller BR, Babetto E. SCG10 is a JNK target in the axonal degeneration pathway. Proceedings of the National Academy of Sciences of the United States of America. 109(52):E3696-705. 2012.
Kynast KL, Russe OQ, Möser CV, Geisslinger G, Niederberger E. Modulation of central nervous system-specific microRNA-124a alters the inflammatory response in the formalin test in mice. Pain. 154(3):368-76. 2013.
Kallenborn-Gerhardt W, Hohmann SW, Syhr KM. Nox2-dependent signaling between macrophages and sensory neurons contributes to neuropathic pain hypersensitivity. Pain. 155(10):2161-70. 2014.
Mukai J, Tamura M, Fénelon K. Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia. Neuron. 86(3):680-95. 2015.
Islam O, Gong X, Rose-John S, Heese K. Interleukin-6 and neural stem cells: more than gliogenesis. Molecular biology of the cell. 20(1):188-99. 2009.
Heine S, Michalakis S, Kallenborn-Gerhardt W. CNGA3: a target of spinal nitric oxide/cGMP signaling and modulator of inflammatory pain hypersensitivity. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(31):11184-92. 2011.

STMN2 antibody

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