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Product Name
STIM2 antibody
- Documents
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Description
STIM2 Rabbit Polyclonal antibody. Positive IHC detected in human spleen tissue, human brain tissue. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells. Observed molecular weight by Western-blot: 85-115 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ39527 antibody; KIAA1482 antibody; STIM2 antibody; stromal interaction molecule 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STIM2 recombinant protein (Accession Number: BC146661). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115708(STIM2 Antibody) at dilution of 1:1000
IP Result of anti-STIM2 (IP:Catalog No:115708, 3ug; Detection:Catalog No:115708 1:500) with HEK-293 cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human spleen using Catalog No:115708(STIM2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:115708(STIM2 antibody) at dilution of 1:100 (under 40x lens)
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Background
STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.
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References
- Lee KJ, Woo JS, Hwang JH. STIM1 negatively regulates Ca²⁺ release from the sarcoplasmic reticulum in skeletal myotubes. The Biochemical journal. 453(2):187-200. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"