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STIM1 antibody

Cat.#: 115707

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Product Information

  • Product Name
    STIM1 antibody
  • Documents
  • Description
    STIM1 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in Jurkat cells, BxPC-3 cells, HT-1080 cells, mouse liver tissue, NIH/3T3 cells, Raji cells. Positive FC detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue, human lung cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 80-90 kDa
  • Tested applications
    ELISA, WB, IP, IHC, IF, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    D11S4896E antibody; GOK antibody; STIM1 antibody; stromal interaction molecule 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of STIM1 recombinant protein (Accession Number: NM_003156). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115707(STIM1 antibody) at dilution of 1:300

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115707(STIM1 antibody) at dilution of 1:300

    IP Result of anti-STIM1 (IP:Catalog No:115707, 3ug; Detection:Catalog No:115707 1:1000) with Raji cells lysate 3800ug.

    IP Result of anti-STIM1 (IP:Catalog No:115707, 3ug; Detection:Catalog No:115707 1:1000) with Raji cells lysate 3800ug.

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115707(STIM1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115707(STIM1 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:115707(STIM1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of HepG2 cells using Catalog No:115707(STIM1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    1X10^6 HepG2 cells were stained with .2ug STIM1 antibody (Catalog No:115707, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with .2ug STIM1 antibody (Catalog No:115707, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER–plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.
  • References
    • Hong JH, Li Q, Kim MS. Polarized but differential localization and recruitment of STIM1, Orai1 and TRPC channels in secretory cells. Traffic (Copenhagen, Denmark). 12(2):232-45. 2011.
    • DebRoy A, Vogel SM, Soni D, Sundivakkam PC, Malik AB, Tiruppathi C. Cooperative signaling via transcription factors NF-κB and AP1/c-Fos mediates endothelial cell STIM1 expression and hyperpermeability in response to endotoxin. The Journal of biological chemistry. 289(35):24188-201. 2014.
    • Wang Y, Chaudhari S, Ren Y, Ma R. Impairment of hepatic nuclear factor-4α binding to the Stim1 promoter contributes to high glucose-induced upregulation of STIM1 expression in glomerular mesangial cells. American journal of physiology. Renal physiology. 308(10):F1135-45. 2015.
    • MacGlashan D. Expression profiling of human basophils: modulation by cytokines and secretagogues. PloS one. 10(5):e0126435. 2015.
    • Zhang H, Sun AY, Kim JJ. STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node. Proceedings of the National Academy of Sciences of the United States of America. 112(41):E5618-27. 2015.
    • Gruszczynska-Biegala J, Pomorski P, Wisniewska MB, Kuznicki J. Differential roles for STIM1 and STIM2 in store-operated calcium entry in rat neurons. PloS one. 6(4):e19285. 2011.
    • Sundivakkam PC, Natarajan V, Malik AB, Tiruppathi C. Store-operated Ca2+ entry (SOCE) induced by protease-activated receptor-1 mediates STIM1 protein phosphorylation to inhibit SOCE in endothelial cells through AMP-activated protein kinase and p38β mitogen-activated protein kinase. The Journal of biological chemistry. 288(23):17030-41. 2013.
    • Chaudhari S, Wu P, Wang Y. High glucose and diabetes enhanced store-operated Ca(2+) entry and increased expression of its signaling proteins in mesangial cells. American journal of physiology. Renal physiology. 306(9):F1069-80. 2014.

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