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Product Name
STAM antibody
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Description
STAM Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, Jurkat cells, Y79 cells. Positive IP detected in PC-3 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686J2352 antibody; STAM antibody; STAM 1 antibody; STAM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STAM recombinant protein (Accession Number: BC030586). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
WB result of Catalog No:(STAM antibody).
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115672(STAM antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using STAM antibody Catalog No:115672 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-STAM (IP:Catalog No:115672, 4ug; Detection:Catalog No:115672 1:300) with PC-3 cells lysate 1440ug.
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Background
STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)
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References
- Malik R, Marchese A. Arrestin-2 interacts with the endosomal sorting complex required for transport machinery to modulate endosomal sorting of CXCR4. Molecular biology of the cell. 21(14):2529-41. 2010.
- Verma R, Marchese A. The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR. The Journal of biological chemistry. 290(11):6810-24. 2015.
- Malik R, Soh UJ, Trejo J, Marchese A. Novel roles for the E3 ubiquitin ligase atrophin-interacting protein 4 and signal transduction adaptor molecule 1 in G protein-coupled receptor signaling. The Journal of biological chemistry. 287(12):9013-27. 2012.
- Holleman J, Marchese A. The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4. Molecular biology of the cell. 25(12):1892-904. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"