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Product Name
SRF antibody
- Documents
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Description
SRF Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 65-70 kDa,40 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MCM1 antibody; Serum response factor antibody; SRF antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SRF recombinant protein (Accession Number: NM_003131). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115583(SRF antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human brain using Catalog No:115583(SRF antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:115583(SRF antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SRF (IP:Catalog No:115583, 3ug; Detection:Catalog No:115583 1:1000) with HeLa cells lysate 3000ug.
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Background
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The calculated molecular weight of SRF is 51 kDa, but the phosphorylated SRF protein is 60-70 kDa.
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References
- Verma S, Lovren F, Pan Y. Pedicled no-touch saphenous vein graft harvest limits vascular smooth muscle cell activation: the PATENT saphenous vein graft study. European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery. 45(4):717-25. 2014.
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