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Product Name
SPRY2 antibody
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Description
SPRY2 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in A375 cells. Positive WB detected in human duodenum tissue, A375 cells. Observed molecular weight by Western-blot: 39kd+60~65kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hSPRY2 antibody; Protein sprouty homolog 2 antibody; sprouty homolog 2 (Drosophila) antibody; Spry 2 antibody; SPRY2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPRY2 recombinant protein (Accession Number: NM_001318538). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human duodenum tissue were subjected to SDS PAGE followed by western blot with Catalog No:115648(SPRY2 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:115648(SPRY2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:115648(SPRY2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A375 cells using Catalog No:115648(SPRY2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Sprouty(SPRY) genes encode intracellular inhibitors of receptor tyrosine kinase (RTK) signaling pathways, including those triggered by fibroblast growth factors (Fgfs). In addition to its well-reported function as an inhibitor of the RAS/RAF/MEK signalling cascade, SPRY2 exerts its inhibitory role in the PI3K/AKT signalling pathway throuth PTEN, and inhibits ERK/MAPK activity via EGFR trafficking.
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