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SPI1 antibody

Cat.#: 115568

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Product Information

  • Product Name
    SPI1 antibody
  • Documents
  • Description
    SPI1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse liver tissue, HepG2 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 31-38 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    31 kDa transforming protein antibody; PU.1 antibody; SFPI1 antibody; SPI 1 antibody; SPI A antibody; SPI1 antibody; Transcription factor PU.1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_003120). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:50-1:500

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115568(SPI1 antibody) at dilution of 1:300

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115568(SPI1 antibody) at dilution of 1:300

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:115568(SPI1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:115568(SPI1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1.
  • References
    • Lin HS, Gong JN, Su R. miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression. Journal of leukocyte biology. 96(6):1023-35. 2014.
    • Shi Y, Xu LZ, Peng K. Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. Scientific reports. 5:17651. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"